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Viant MR Pincetich CA Walton JH Tjeerdema RS Hinton DE 《Marine environmental research》2002,54(3-5):553-557
In vivo nuclear magnetic resonance spectroscopy (NMR) is a powerful technique for characterizing the sublethal actions of physical and chemical stressors in live, intact organisms. In particular, 31P NMR is ideal for observing perturbations to cellular energetics since critical metabolite concentrations, including phosphagens, ATP and inorganic phosphate (Pi), can be measured non-invasively and in real time. This technique's versatility is demonstrated not only in the diversity of organisms that can be studied, but also in its broad-ranging applicability to environmental research. Illustrative studies include the actions of copper in adult red abalone (Haliotis rufescens) and changes in energetically important metabolites in developing medaka embryos (Oryzias latipes). Advantages and disadvantages of in vivo NMR will be discussed. 相似文献
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A field experiment was carried out in Hong Kong to study the patterns of recolonization and succession of subtidal macrobenthos in defaunated sediment contaminated with industrial wastes and to determine the time required for benthic recovery in the industrial-contaminated sediment. A total of 50 species was found with an average of 172 animals/tray and 24 species/tray recorded one month after deployment. Initial colonizers were predominantly polychaetes (96 animals/tray, accounting for 55.7%) and gastropods (47 animals/tray, accounting for 27.2%). Abundance of macrobenthos increased quickly to a peak (505 animals/tray) after four months, declined afterwards, and increased again till the end of the experiment. Species number peaked (57 species/tray) in the same month as abundance did, and gradually declined thereafter. Abundance, species number and diversity were significantly lower in the industrial-contaminated sediment as compared to the controls during the early successional stages, indicating the harmful effects of industrial wastes on recolonization and succession of macrobenthos. Although no significant differences in community parameters between the industrial-contaminated and the control sediments were found after eleven months, significant difference in species composition still existed after fourteen months, showing a relatively long-term impact of industrial wastes on macrobenthic community structure. 相似文献
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卡尼湾干谷的钼矿床以网状脉化和矽卡岩化为特征。然而不同的围岩有明显不同的矿物组合。在卡尼湾岩株中,辉钼矿产于典型的石英-钾长石或石英-白云母脉中。在围岩中,辉钼矿产于绿泥石+黄铁矿+磁铁矿中,其中包含有不同量的石英、方解石和绿帘石。包含于石英和萤石中的原始流体包裹体有水溶液、液体CO2和气体CO2。 相似文献
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文章译自美国国家海洋大气局(NOAA)、国家环境卫星数据及服务局(NESDIS)局长Robert S.Winokur的报告。文章介绍了迈向21世纪的美国诺阿环境卫星坟墓和卫星遥感应用技术的进展,使读者了解未来美国极轨气象卫星系统、极具前景的三维温,湿变量卫星遥感反演以及静止气象卫星最新遥感应用技术发展的前景。 相似文献
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Analysis of the 16S-23S rDNA intergenic spacers (IGSs) of marine vibrios for species-specific signature DNA sequences 总被引:7,自引:0,他引:7
Vibrios are widespread in the marine environment and a few pathogenic species are known to be commonly associated with outbreaks of diarrheal diseases in humans due to the consumption of raw or improperly cooked seafood. However, there are also many Vibrio species which are potentially pathogenic to vertebrate and invertebrate aquatic animals, and of which little is known. In an attempt to develop rapid PCR detection methods for these latter class of vibrios, we have examined the 16S-23S intergenic spacers (IGSs) of 10 lesser-known Vibrio species and successfully developed species-specific primers for eight of them--Vibrio costicola, V. diazotrophicus, V. fluvialis, V. nigripulchritudo, V. proteolyticus, V. salmonicida, V. splendidus and V. tubiashii. The IGS amplicons were amplified using primers complementary to conserved regions of the 16S and 23S rRNA genes, and cloned into plasmid vectors and sequenced. Analysis of the IGS sequences showed that 37 ribosomal RNA (rrn) operons representing seven different IGS types have been cloned from the 10 vibrios. The three IGS types--IGS(0), IGS(IA) and IGS(Glu)--were the most prevalent forms detected. Multiple alignment of representative sequences of these three IGS types from different Vibrio species revealed several domains of high sequence variability, which were used to design species-specific primers for PCR. The specificity of the primers were evaluated using total DNA prepared from different Vibrio species and bacterial genera. The results showed that the PCR method can be used to reliably detect eight of the 10 Vibrio species in marine waters in this study. 相似文献
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利用1989 ̄1994年的地震数据,对Nishenko根据地震空区理论所作的预测进行了检验。由于预报是以考虑了板块边缘各段特有的特征震级和复杂时间的首次全球性地震预测,故称之为“新的地震空区”推测。Nishenko的预报给出了从1989年第一天开始,计算时估分别在5a、10a和20a内将会被特征震级填充的约100个地震区各自的发震概率值。目前仅可能对这些首次的预报加以经验。我们所利用的3个检验依据 相似文献
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